Bursaphelenchus xylophilus detector

1.Uses of Bursaphelenchus xylophilus detector instruments

Bursaphelenchus xylophilus detector uses fluorescent polymerase chain reaction to analyze pine wood nematode genes and can be detected and identified in trees in the open air.Bursaphelenchus xylophilus detector mainly includes the isolation, DNA extraction, fluorescence quantitative PCR detection and detection of pine nematodes.The detection work of pine nematodes can be quickly completed, the detection results can be automatically displayed and the detection report is printed, without manual interpretation, and the accuracy rate is greater than 99%, greatly improving the efficiency of pine nematode detection work.The field quick inspection system can be equipped with portable and mobile operations, providing convenience for wild pine nematode detection.The automated molecular detection system of pine nematode is widely used in Forestry Bureau, Forest Defense Station, Quarantine Bureau, Customs, University, Scientific Research Institution and other units.

2.Features of Bursaphelenchus xylophilus detector instruments

1.High accuracy of system detection.

2.The intelligent pine nematode DNA rapid detection system has built-in pine nematode DNA automated detection program, which does not require complicated program settings and runs automatically with one-click.It can not only detect nematode isolation liquid, but also directly detect pine wood chips without separating nematodes.

3.The system inspection operation is simple to operate, time-saving and efficient, and is suitable for forestry and forest defense departments at all levels.The entire process including sample processing, DNA extraction, on-machine detection and result interpretation can be completed in as fast as 60 minutes.

4.Using amplification fluorescence technology, the laboratory testing and identification requirements can be completed through the system host.

5.Small size, light weight, easy to carry.Easily meet the needs of going out experiments.

6.Built-in 10-inch high-definition capacitor screen PDA, touch screen operation, simple and fast.

7.Marlow's high-quality Peltier refrigeration plate, combined with Germany's high-end PT1000 temperature sensor and the temperature control mode of the electrical resistance heating compensation edge, the maximum temperature increase speed is 7℃ and the maximum temperature decrease speed is 5℃, greatly shortening the experimental time.

8.The 3s fast lighting mode of the whole plate ensures consistency of the hole position of the experimental results.

9.Concise and intuitive software guidance, easily enable detection experiments.

3.Bursaphelenchus xylophilus detector technical parameters

Sample capacity: 48x0.2ml, support 8-connection pipe

Suitable consumables: Common transparent PCR consumables, 8x0.2ml drain pipe, 0.2ml single tube

Reaction system: 5-120ul reaction mode system

Heating/refrigeration module: high-performance imported semiconductor thermoelectric module

Temperature control range: 4°C-99°C

Hot cover temperature: 30℃-108℃ (default 105℃, hot cover temperature can be adjusted)

Warming rate: 3.5℃/s (MAX)

Cooling rate: 2.5℃/s (MAX)

Temperature control accuracy: ±0.1°C

Temperature uniformity: ±0.25°C

Temperature accuracy: ±0.1℃

Number of temperature control areas: multiple points (2 points)

Number of gradients: 0

Gradient temperature range: None

Gradient hole number: None

Excitation light source: high power LED (maintenance-free, long life)

Excitation light wavelength range: 400-700nm

Detection components: imported high sensitivity photoelectric sensor

Excitation and detection of propagation medium: high temperature resistant professional optical fiber

Number of detection channels: 4 channels are standard

Applicable dyes and probes:

First channel: 470/520 FAM, SYBR Green

Second channel: 530/570 HEX, JOE, VIC

Third channel: 580/610 ROX, CY3.5, Texas-Red

Channel 4: 630/670 CY5

Software function: Multiple qPCR data analysis function, can detect 4 detection channels simultaneously

amplification curve,

Standard curve quantification,

Melting curve,

Support HRM detection

Data export: The experimental results can be directly exported in the form of graphs and tables; the detailed data report content includes experimental conditions, data coordinates and tables, and data analysis parameters.

Data format: XLS, PDF, DOCX

Noise: <45dB

Screen size: 10 inches (HD)

Touch screen: capacitive

External USB: Supports data import and export

Heat cover: automatic pressure adjustment

4.Bursaphelenchus xylophilus detector configuration list